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To establish a linear curve for the detection of MNA peak, a 10-0.3125 uM/100 L half-fold serially diluted samples of MNA were prepared in reaction buffer containing 1 mM Tris [pH 8.6], 1 mM DTT, 10% trichloroacetic acid, 4% methanol, and water

Trop Med Infect Dis 8:125

Nerve targets sit at the far end for this reason

Vitamin B12 deficiency can lead to mild or even serious health issues, but the good news is that its treatable, often through dietary changes, supplements, and lifestyle adjustments