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Immunohistochemical staining After dewaxing, rehydration, and antigen retrieval, tissue sections were treated with 3% H 2 O 2 to block endogenous peroxidase activity and then blocked with 5% goat serum

The rebound of renal Cys levels in young and mature mice after 24 hr may indicate that hepatic efflux has returned to baseline levels, while the reduction of Cys in old mice may reflect a lower rate of GSH synthesis and turnover, possibly as a result of fasting as previously observed in old animals [64]

Wang H, Nie J, Li P, Zhang X, Wang Y, Zhang W, et al

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