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Iron metabolism, in turn, affects immune homeostasis through a variety of complex mechanisms, including promoting toxic stress, inducing inflammatory responses, and damaging immune cells, leading to the development of RA

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Cells were mixed with transfection reagents and plated at 13,000 cells per well into 96-well Greiner CELLSTAR white-walled plates (Sigma-Aldrich), and assays were performed 48 h later