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Serum was separated and stored at 80C and used later for enzyme-linked immunosorbent assay (ELISA)

The Ct values were obtained from Ct values of interest genes subtracting that of loading control

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In vivo ubiquitylation assay HEK293 cells were co-transfected with the indicated plasmids or siRNA oligos for 48 h, and then treated with relevant activators or inhibitors

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