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One review concluded that vitamin B12 plays a key role in fat metabolism, noting that a deficiency could be linked to increased fat accumulation and obesity ( Another animal study found that severe vitamin B12 deficiency led to a significant increase in body fat percentage and cholesterol levels in pregnant mice ( Whats more, one animal study showed that administering B vitamins to rats fed a high fat diet reduced weight gain and increased levels of several enzymes that influence metabolism, compared with a control group ( However, current evidence is lacking, and additional high quality studies are needed to determine how vitamin B12 may affect metabolism and body fat in humans

A SYBR Premix Ex Taq II (Tli RNaseH Plus) Kit was used to perform qRT-PCR in technical triplicate on a StepOnePlus Real-time PCR System (Applied Biosystems, USA) following the manufacturers instructions

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9,12-15 A systematic review and meta-analysis of randomised controlled trials evaluating weight reduction diets, with or without exercise, reported an 18% reduction in all-cause mortality over a median follow up of two years in adults with obesity

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