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Then, the cells were incubated on ice in a 1 : 10 PBS mobile phase solution containing metaphosphoric acid 5% for 30 min

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Furthermore, our enzyme kinetic assays showed that although 6-hydroxy-FAD-reconstituted hFSP1 exhibited lower affinities ( K m ) for NADH and CoQ 1 than FAD-reconstituted hFSP1, the NADH oxidation activity ( k cat ) of 6-hydroxy-FAD-reconstituted hFSP1 was higher than that of FAD-reconstituted hFSP1 (Supplementary Table 3)

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