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Standard subcutaneous protocols deliver 250 to 500 mcg per day, dosed once or twice daily on an empty stomach (food blunts the lipolytic effect)

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To obtain bacteriological sterility prior to conducting immunopeptidomics experiments, the cell pellet was lysed using 2x lysis buffer and complete protease inhibitor cocktail (Roche) for 30 minutes on ice, with periodic shaking every 10 minutes

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