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Supernatant was discarded, and the cell pellets were washed with wash buffer (10 mM Tris-HCl, pH = 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA with protease inhibitors) and resuspended in the sonication buffer (10 mM Tris-HCl, pH = 8.0, 1 mM EDTA, 0.1% SDS with protease inhibitors)

EAU guidelines have recommended a series of evaluation as a routine part of the initial assessment of men with LUTS, that includes clinical history, a validated questionnaire to assess symptoms, physical examination, creatinine measurement, urinalysis, flow rates, postvoid residual (PVR) volume and serum prostate-specific antigen (PSA) measurement especially when a diagnosis of prostatic carcinoma is required [9]

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