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Our BAC-transgenic based methodology with systematic deletions (=subtraction of gene regulatory modules) in bacterial cells might complement the inventive Kokubus method and would thus provide ideal foundations in assessment of any detailed gene regulatory landscapes most of which remain elusive

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& Callard, G

2.5, Thermo Scientific) with implemented SEQUEST and Amanda algorithm

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Conversely, [U- 13 C]glucose tracing showed GGCT overexpression enhanced Glc-derived carbon flux into the TCA cycle (increased m + 2 labeling of succinate, fumarate, malate), which was reversed by the E98A mutant (Fig