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The authors would like to note that the actual size of glutathione in a non-aggregated state is outside of 0.87 nm detection limit for what can be measured accurately by the dynamic light scattering technique

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Therefore, we designed an SH-free Ec CutA1 mutant, referred to as Ec CutA1_0SH (Cys16 Ala, Cys39 Ala, Cys79 Ala), with the aim of achieving high heat reversibility

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10.1006/meth.2001.1262 58 Lopez-GonzalezR.LuY.GendronT

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