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The cross sectional study design, however, did not allow for such a time-ordered analysis, and follow-up studies where researchers measure individuals at multiple points in time (e.g., longitudinal) are needed to better test the proposed theory of why some individuals develop more marijuana related consequences than others

HPLC and mass spectrometry were carried out following previous methods, with modifications ( Monoamine oxidase A activity, qRT-pCR and dopamine content ELISA kit The striatum of mice was excised with a mold in a buffer solution at 4C, quickly frozen in liquid nitrogen, and stored at 80C

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