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Animals were anaesthetized with ethyl ether after which a single intracolonic dose of 30 mg of TNBS dissolved in 0.25 ml of either 50% ethanol in saline (v/v) or saline, referred as TNBS+ethanol or TNBS+saline, respectively, was administered

doi: 10.1016/j.pneurobio.2020.101973 62 GaciasM.GaspariS.SantosP

The early activation of PI3K strongly enhances the resistance of cortical neurons to hypoxic injury via the activation of downstream targets of the PI3K pathway and the normalization of the levels of PARP activity, ATP, and NAD+

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Moreover, the increase in the percentages of PI positive cells was significantly enhanced by erastin (Figures 1 F, G), but reversed by Fer-1

Still, it suggested the mechanism was working in humans, not just mice