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Tretinoin has been shown to activate the cGAS/STING pathway and inhibit oocyte maturation by inducing mtDNA leakage [309, 310]

Reverse transcription quantitative polymerase chain reaction Complementary DNA (cDNA) was synthesized from 1 g of RNA using M-MuLV Reverse Transcriptase (New England Biolabs Inc., MA, USA) according to the manufacturers protocol