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The TileSeq sequence analysis package (used to calculate relative read frequencies in the pre-and post-selection libraries) can be found on github at The MAVE scoring function can be found on github at Abbreviations aa: Amino acids AdoMet: S -Adenosylmethionine, substrate for many cellular methylation reactions and binding partner for the CBS regulatory domain, also abbreviated as SAM AUROC: Area under the precision-recall curve CBS: Cystathionine -synthase C-terminus: Carboxyl-group terminus of a protein sequence CYS4 : Cysteine auxotrophy gene 4, the yeast orthologue of CBS dNTP: Deoxy-ribonucleoside 5-triphosphate dUTP: Deoxy-uridine 5-triphosphate E

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Additional outcomes include assessing number of days with fever, and to assess symptoms, including dyspnea and fatigue