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Mitochondria-targeted monofunctional platinum complexes can accumulate in the mitochondria, induce significant changes in mitochondrial ultrastructure and membrane, release cytochrome c(Cytc) from mitochondria, and disrupt mitochondrial OXPHOS and glycolysis (118)

After 30 minutes of incubation, lysates were centrifuged at 12,000 g , and the supernatants were collected for protein estimation and immunoblotting

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Injection Technique General subcutaneous technique, following established clinical best-practice guidance [14]