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Sites : Abdomen (most common) Front of thigh Back of upper arm Process : Clean site with alcohol Draw dose into insulin syringe Pinch skin Insert at 45-90 degree angle Inject slowly Dispose needle safely Timing : Morning preferred (supports daytime tissue repair) Rotating sites : Prevents tissue damage Protocol duration Acute inflammation : 6-8 weeks minimum Skin/anti-aging : 12-24 weeks for significant results Chronic conditions : 12+ weeks, may use long-term Maintenance : Some use 1mg 3-4x weekly after initial protocol Cycling : Not required

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These interactions may underpin its potential influence on neurotransmission, neural excitability, and stress adaptation

doi: 10.1210/jc.2004-0039 36 SakaiKDErcoleAJMurphyLJClemmonsDR

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