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After treatment with different regents, both floating cells and adherent cells were harvested and stained with 5 g/ml PI (Sigma-Aldrich P486) immediately before flow cytometric analysis

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Interestingly, polymorphisms in genes encoding complement factors implicated in the risk of AMD and progression to GA do not necessarily influence the expansion of GA lesions and, in some cases, are even associated with slower lesion enlargement [24]