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Without enough of it, you may become anemic leading to fatigue, shortness of breath, dizziness, and complications during labor and delivery [*]

The behavior of hydrophilic and hydrophobic bases further complicates scale-up: Hydrophilic bases (e.g., PEG) solidify differently than lipophilic ones (e.g., cocoa butter or semi-synthetic glycerides), requiring tailored cooling profiles and mold handling

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Understanding these differences is crucial for designing meaningful experiments

Post transfer, membranes were stained with amido black (0.1% w/v Amido black, 10% v/v acetic acid and 25% v/v isopropanol) to monitor protein transfer, then de-stained 10% v/v acetic acid and 25% v/v isopropanol

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