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Injection of IL-6 in healthy subjects leads to activation of the HPA system, increased cortisol production, breakdown of fat stores, and greater energy expenditure

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JAMA Psychiatry 82 , 4756 (2025)

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Accordingly, several in vitro studies aimed at assessing the efficacy of drugs on glucose-related cell damage in ARPE-19 reported that using euglycemic media (5.5 mM) as a control condition may affect normal cell growth [48]

For systemic peptides like MOTS-c or SS-31 , the injection site matters less because the peptide enters systemic circulation regardless of where it is administered