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Background corrections were performed for XPD, Fancc, GR, 8-oxo-dG and Idh1 as follows: (1) two regions of interest (ROIs) were selected from the summed z-stack (50 50 pixels in dimension and in areas of tissue coverage), (2) these intensity values were divided by area (accounting for number of z-stacks) and then averaged generating a mean background value

10.1016/j.brainres.2013.01.007 58 KimY.-M.KimS.-J.TatsunamiR.YamamuraH.FukaiT.Ushio-FukaiM

Patient education is key