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Puerarin improves the bone micro-environment to inhibit OVX-induced osteoporosis via modulating SCFAs released by the gut microbiota and repairing intestinal mucosal integrity

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Co-immunoprecipitation The recombinant GST-tagged Nanobody against GFP 69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to the manufacturers instructions

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For in vitro experiment, 5000 cells were seeded in a 96-well white plate and incubated in normal culture conditions for 24 h

Depletion of Trim26 in hepatocytes alters liver metabolic equilibrium, leading to glucose metabolic dysfunction, lipid buildup, hepatic inflammation, and fibrosis, all of which accelerate the progression of the NASH phenotype [42]