Last updated: May 2026
To the right are widefield images of HeLa cells with lentivirus mediated LUC, Lb NOX, Ec STH, mito Ec STH expression under Dox control transiently expressing SoNar, in basal medium (DMEM without pyruvate, fluorescent vitamins and phenol red with 5 mM glucose, 25 mM HEPES, pH 7.4 and 1% dialyzed FBS), or after addition of 1 M Ant A, or when cells were switched to the basal medium without glucose but with 10 mM pyruvate
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RLS can begin at any age, although many people who exhibit the symptoms of RLS are middle-aged or older
After the initial incubation, 0.5 mL of 1% starch solution was added to the reaction mixture and incubated further for 10 min
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