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Furthermore, while 4-HNE promotes cyst growth via multiple kinase pathways (270), the hierarchical integration of these pathways (e.g., which is the rate-limiting step) and their crosstalk with ferroptosis core networks (e.g., lipid peroxidation amplification) are poorly defined

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The mixture was placed into a 1 mL cuvette and read with Shimadzu UVPC 2100 spectrophotometer set at 366 nm at 37C