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92% have a PCR technique for HBV/HCV

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In recent years, researchers have also developed different strategies to alleviate hypoxia within the tumor, such as oxygen delivery to the tumor region [38, 39] and in situ oxygen generation [40,41,42] in the tumor region

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For fluorescence microscopy studies, HL-60 cells and HepG2 cells (seeding densities 1 10 4 cells/well and 8 10 4 cells/well, respectively) were placed in serum-free medium to facilitate their adhesion to coverslips and cultured on lysine-coated 8-well chamber slides (IBIDI) following the procedure detailed by 2.8 Cell surface binding, endocytosis, and downregulation of TfRs For surface binding and endocytosis of AF-labeled proteins, the cells were rinsed twice with ice-cold HEPES-buffered, serum-free media, pH 7.4, and incubated for 1 h on ice with either 500 nM hemeAF546HPX, hemeAF546HPX, or AF488 holo-human Tf

[PubMed] [Google Scholar] 13.Meister A