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1 Evaluating a metabolic acidosis disorder must always include calculation of the unmeasured anions present in serum, including proteins, phosphate, sulfate, and organic anions

The inventors have found that in some embodiments the inclusion of an extraneous or exogenous thiol as an additive in the reaction mixture reduces the formation of undesirable by products

doi: 10.3390/biom11040589 87 YoussefLARebbaaAPampouSWeisbergSPStockwellBRHodEAet al

Statistical analysis The statistical evaluation of the data began by validating the normal distribution of all variables using the Shapiro-Wilk normality test

Nightingale, K