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Immunology: Magnetic beads are ideal for isolating antibodies, antigens, and immune complexes

After 96 h, wells were aspirated, washed with PBS, and a fixative/staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399) was added

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Am J Physiology-Regulatory Integr Comp Physiol

After drying, a 100 l sample diluent was added to each well and incubated on a shaker at room temperature for 1 h to block the protein chip