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Description

The therapy aims to enhance the bodys natural healing processes by increasing oxygenation, reducing inflammation, and boosting the immune system

546 Inspiringly, first, from the Comprehensive Assessment of Long-term Effects of Reducing the Intake of Energy (CALERIE) trial, CR slows ageing in healthy adults by 23%, as measured by a DNA methylation biomarker for the pace of ageing calculated from the epigenome (DunedinPACE)

The RTqPCR mixture consisted of 5 L of SYBR Green Master Mix (Yeasen, Shanghai, China), 1 L of upstream primer, 1 L of downstream primer (with a final concentration of 0.2 M for each primer), and 2 L of cDNA, with enzyme-free water added to a final volume of 10 L