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Moreover, the interaction of -SNAP through this hydrophobic loop is critical for its canonical and non-canonical functions as mutations in this loop reduce by ~25 times the efficiency to disassemble SNAREs complexes 11 and negatively regulate SOCE 6

You end up with a solution that might look right but has a fraction of its expected biological activity

Molecules like the cell cycle protein p21 have been reported to interact directly with GPX4, competitively or allosterically affecting its ubiquitination rate, thereby stabilizing GPX4 and enhancing cellular resistance under oxidative stress (Zheng et al., 2024)