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Cultures were pulse-labeled with 10 M BrdU (a nucleoside analog that competes with thymidine for incorporation into DNA) in DMEM-10% HS for the final 2-h period of 48-h in culture, followed by immunocytochemistry for detection of BrdU using G3G4 anti-BrdU mAb (1:100 dilution) and HRP-conjugated anti-mouse IgG Ab (1:500 dilution)

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PUFA-PEs promote intracellular lipid peroxide accumulation under the action of various enzymes

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In addition, they keep a clear line between discussing how a study is structured and making claims about real-world effects, especially when peptides are labeled strictly for research use only and not for human consumption

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