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Melting curves were fitted in Origin Pro 9 software using the Boltzmann sigmoidal equation F = F min + ( F max F min )/(1 + exp(( T t T )/ s )), where F refers to fluorescence ( = 660 nm) and F min and F max are the minimum and maximum fluorescence, respectively, T is temperature, T t is the transition temperature, and s is a fitting parameter

Exome sequencing in amyotrophic lateral sclerosis identifies risk genes and pathways

doi: 10.1002/aur.2617 57 LeiterO.ZhuoZ.RustR.WasielewskaJ

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These findings were further validated through co-autoxidation experiments with egg phosphatidylcholine and STY-BODIPY, using a lipophilic alkoxyl radical generator

After incubation, the cells were washed twice with PBS (1X, pH 7.4), and total cellular proteins were extracted using a lysis buffer containing EDTA (10 mM), TrisHCl (50 mM