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Methylene blue is commonly used to test the integrity of the gastrointestinal tract following anastomosis.1 This substance is also used for diagnostic tests such as localisation of parathyroid adenoma during surgery and identification of fistula tracts, as a therapeutic agent for the treatment of methemoglobinaemia or refractory hypotension and as a mild antimicrobial agent

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In another 2006 clinical trial, (10) 72 test subjects with pressure ulcers were presented with TB-500