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The plastidial localization of AtSufB was confirmed with confocal microscopic analysis of onion epidermal cells expressing the AtSufB-GFP fusion protein (Mller et al., 2001)

doi: 10.2147/PHMT.S129362

Consequently, the DNA polymerase enables the addition of the correct nucleotide to the incision under the guidance of the opposite strand before the DNA ligase finally seals the entire backbone