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Lysates were incubated on ice for 30 minutes with occasional vortexing, and centrifuged at 14,000 g for 15 minutes at 4C

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Although postload homocysteine measurements were not carried out, the stronger relationship in women might be partially caused by a more efficient transsulfuration in men, who present a lower response to the methionine overload than women at age-specific ranges (Silberberg et al, 1997)