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Novel small molecule agonist of TGR5 possesses anti-diabetic effects but causes gallbladder filling in mice

The gRNA target (5-CCT TTG ACC GAG CCC ATA TC-3) and single-stranded DNA oligonucleotide (ssODN) donor (5-AGC AGC TCC ACC AAG CAG GAT GGG CAG GAA CAG TTA GTG GTG GGG CAC AAT GTT TCC TTT GCC CGA GCC CAT ATC AGA GAA CAG TAT CTG ATT CAG GTA AGG TTT GTG GGA ATG GAG TGC TGT AAC ATA AGG TGG GCC-3) were synthesized (Integrated DNA Technologies)

Then, according to the required dosage and concentration, use a syringe to extract an appropriate amount of sterile water or physiological saline, and slowly inject it into the gonadorel powder bottle

Eur Arch Psychiatry Clin NeuroSci

Acknowledgements This work was partially funded by the Italian Ministry of Education (MIUR, Grant to GS) and by Sigma Tau Industrie Farmaceutiche Riunite (Grant to GR)