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Liver homogenates (10 L) were protein-precipitated with nine volumes of acetonitrile/methanol/formic acid (74.9:24.9:0.2, v/v/v) containing stable isotope-labeled internal standards (valine-d8 and phenylalanine-d8)

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Localization of a novel locus for autosomal recessive early-onset parkinsonism, PARK6, on human chromosome 1p35-p36

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Most sessions last between 30-60 minutes, depending on the specific formulation and dosage