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After thawing, cells were filtered through EASYstrainer 40 m (Greiner), washed with PBS and cultivated in RPMI-1640 (Gibco) supplemented with 10% fetal bovine serum (FBS-12A, Capricorn Scientific), 200 U/mL penicillin and 200 g/mL streptomycin (Gibco)

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