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The experiment was conducted following the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines, the European Community Guidelines (Directive 2010/63/EU), and the normative resolutions issued by the National Research Council's Guide for the Care and Use of Laboratory Animals, as well as the National Council for the Control of Animal Experimentation (CONCEA), the Brazilian Practice Guideline for the Care and Use of Animals for Scientific and Didactic Purposes (DBCA), and the Guidelines for the Practice of Euthanasia recommended by CONCEA

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Food production technology should also be improved to mitigate and combat the effects of metal toxicity