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Dietary choline is initially metabolized by the gut microbiota into trimethylamine (TMA), which is then absorbed and further oxidized in the liver into TMAO

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After TGF-1 stimulated for 12 h, cells were stained with C11-BODIPY (581/591) at 37 C for 30 min

Porru M, Zappavigna S, Salzano G, Luce A, Stoppacciaro A, Balestrieri ML, Artuso S, Lusa S, De Rosa G, Leonetti C, Caraglia M (2014) Medical treatment of orthotopic glioblastoma with transferrin-conjugated nanoparticles encapsulating zoledronic acid