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Real-time quantitative polymerase chain reaction Total RNA was extracted from cells using the Trizol reagent (Takara Bio Inc, Japan) according to the manufacturers instructions

Indeed, because more than 100 proteins have been reported as TG2 substrates and because TG2 has broad substrate specificity, the GST tag could be crosslinked with a number of cellular proteins 21

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doi: 10.1016/j.clim.2013.01.001 76 BererKMuesMKoutrolosMRasbiZABozikiMJohnerCet al

As a result, iron accumulates in tissues and organs, which can disrupt their normal functions

Examples: Weight loss plateau scenario: 5% additional fat loss preserved lean mass improved metabolic markers Injury recovery scenario: improved functional capacity pain reduction improved rehabilitation performance Without predefined outcome metrics, stacking becomes experimentation