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HOSu N-hydroxysuccinimide HBt N-hydroxybenzotriazol 1-Hydroxy-7-azabenzotriazol 1-Hydroxy-7-azabenzotriazol 6-chloro-N-hydroxybenzotriazol Hydroxy-3,4-dihydro-4-oxo-1,2,3-benzotriazine PNP p-nitrophenol carboxylic acids activated with an uronium salt or a phosphonium salt such as but not limited to, O-Benzotriazole-N,N,N,N-tetramethyluronium hexafluorophosphate (HBTU), O-(Benzotriazol-1-yl)-N,N,N,N-tetramethyluronium tetrafluoroborate (TBTU), 2-(6-Chloro-1H-benzotriazole-1,1,3,3-tetramethylaminium hexafluorophosphate (HCTU), 2-(6-Chloro-1H-benzotriazole-1-yl)-1,1,3,3-tetramethylaminium tetrafluoroborate (TCTU), 2-(1H-7-Azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU), 2-(3,4- activated acylating agents include, but are not limited to, esters of N-hydroxysuccinimide (NHS ester), p-nitrophenol (PNP ester), N-Hydroxy-5-norbornene-2,3-dicarboxylimide (HONB-ester), N-pentafluorophenol ester (PfP-ester), 2,4-dinitrophenyl ester, 4-nitrophenyl ester, 3-hydroxy-3,4-dihydro-4-oxo-1,2,3-benzotriazine (HODhbt), carbonyldiimidazole (CDl) or N-ethyl-5-phenylisoxazolium-3-sulfonate (NEPIS), preferably a N-hydroxysuccinimide ester, p-nitrophenol or a HOBt ester or a derivative thereof using reaction conditions as, e.g., described in Organic Synthesis on solid Phase (Florencio Zaragoza Dorwald, N-acylated derivatives of carnosine can also be used to obtain N-acylated derivatives of other peptides, including but not limited to, the tri-peptide GHK, GHK esters, the tetra-peptide GEKG (SEQ ID NO:1), and GEKG (SEQ ID NO:1) esters

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Glutathione concentration = [(OD SAMPLE OD BLANK ) / (OD CALIBRATOR OD BLANK )] 100 n (M), where n is the dilution factor (20 for blood samples)

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