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Depending on the experiment, a culture of 100 mL or of 2 L Luria-Bertani (LB) medium supplemented with 100 g/mL ampicillin (LB/Amp) was inoculated with 1/100 volume of an overnight culture and grown at 30 C to OD 600 0.4

Then, samples were centrifuged at 3660 g for 10 min and 150 L of the supernatant were transferred to a new microcentrifuge tube for dry vacuum

In addition, the treatment of leukemic blasts with each of the two extracts combined with some drugs increases the sensitivity to death even in tumor cells that, in some cases, do not respond to conventional therapies

A mutation in the TRPC6 cation channel causes familial focal segmental glomerulosclerosis

J Cyst Fibros 16:214221

False positives for anti-tTG normally display a low antibody titer (less than twice the cut off)