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Mouse tumour models For the non-adipose and iWAT tumour models, MC38 cells (2 10 6 ), DLD1 cells (2 10 6 ) or CT26 cells (1 10 6 ) in 100 l of PBS were subcutaneously injected into the dorsal flank of C57BL/6J, BALB/c nude or BALB/c mice to generate the first generation of cell-derived xenograft tumours

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For histological examinations, sections were stained with hematoxylin and eosin, mounted using DPX mountant (Sigma-Aldrich, USA, Cat

coli DH5-alpha with glycine biosensor (3), GcvA gene construct (4), GcvR gene construct (5) and PepD gene construct (6)

Application of pressurized carbon dioxide during salt-reduced sardine fish sauce production

Gupta M, Sinha S, Chandra P (1996) Copper-induced toxicity in aquatic macrophyte, Hydrilla verticillata: effect of pH