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Targeting glutamine metabolism to enhance Immunoprevention of EGFR-driven lung cancer

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HEK293 cells were lysed in CSK Buffer (10 mM Pipes pH 7.0, 300 mM sucrose, 100 mM NaCl, 3 mM MgCl 2 ) supplemented with 0.1% Triton X-100, Roche Complete EDTA-free protease inhibitor tablet (#11 873 580 001), and Sigma phosphatase inhibitor cocktail 3 (#P0044) for 30 min on ice

Step 3: Massage gently in circular motions with your hands until fully absorbed

Mitochondria are the primary intracellular site of oxygen consumption and the major source of ROS, most of them originating from the ETC