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With a fixed amount of immobilized antigen, the amount of labelled antibody bound decreases as the concentration of unlabelled antigen is increased, allowing quantification of unlabelled antigen ( competitive ELISA )

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The reduction of DHLA-Ala- Au 3+ Cl 4 complexes was complete to form DHLA-Ala@AuNPs in 2 min because DHLA-Ala ligand slightly undergoes deprotonation

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