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doi: 10.1074/jbc.M109014200 47 Perez-EscuredoJDadhichRKDhupSCacaceAVan HeeVFDe SaedeleerCJet al

It changes metabolic signaling and appetite regulation

To address this, we utilized the FISAP platform 33 , which incorporates a custom C18 tip loaded with streptavidin beads (Fig

Validation of the CAchexia SCOre (CASCO)

The proteins were transferred onto a nitrocellulose membrane using a iBlot 2 transfer device (Thermo Fisher Scientific, Waltham, MA, USA), followed by blocking for 90 min in Tris-buffered saline-Tween (TBS-Tween) (50 mM Tris, 0.15 M NaCl and 0.1% Tween-20, pH 7.5) containing 5% milk