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Vinculin run on the same gel was used as a loading control

For increased sensitivity, data was also acquired using positive mode with single ion monitoring for TML and d9-TML, m/z 189 4 and 198.22 4, respectively, at a resolution of 7,500

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Under normal conditions, TMAO is rapidly removed from the circulation, largely via excretion in the urine [56,57]

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