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[181] From an early age, Garland had been billed as "the little girl with the leather lungs," [183] a designation the singer later admitted to having felt humiliated by because she would have much preferred to have been known to audiences as a "pretty" or "nice little girl." [185] Jessel recalled that even at only 12 years old, Garland's singing voice resembled that of "a woman with a heart that had been hurt." [183] The Kansas City Star contributor Robert Trussell cited Garland's singing voice as one of the reasons that her role in The Wizard of Oz remains memorable, writing that although "[s]he might have been made up and costumed to look like a little girl

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Nave CD4 + T cells (0.4 10 6 ) were cultured in RPMI-1640 medium supplemented with 10% FBS (Gibco, CA, USA), 2 mM glutamine, 1% penicillin streptomycin (Gibco, CA, USA), 10 mmol/L N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES, Gibco, CA, USA), and 55 mmol/L 2-mercaptoethanol (Gibco, CA, USA) in 24-well plates

The outlook depends on the severity of the burn is and how quickly treatment is given