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Cell viability, glucose-stimulated insulin secretion, cellular adenosine triphosphate, reactive oxygen species (ROS) levels and Rho kinase (ROCK) activities were measured

Two arginyl residues present at the C-terminus of EcGGT were mutated with alanine (R513A) and glycine (R571G), resulting in a complete blockage of autocatalytic processing (Hashimoto et al., 1992)

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Features were sorted to find the metabolites and lipids with the most relationships with questionnaire items

Concurrently, mtROS stimulates lipid peroxidation, ultimately leading to disruption of mitochondrial membrane potential and mitochondrial calcium buffering capacity (36)