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Briefly, cells were lysed 48 h after transfection in 0.25 M D-mannitol, 0.05 M Tris base, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, and 1% Triton X-100 supplemented with protease and phosphatase inhibitor cocktail tablets (Roche Diagnostics, Mannheim, Germany)

Potential Risks And How To Mitigate Them While glutathione is generally considered safe, especially when obtained from dietary sources, supplements can sometimes pose risks or side effects

Identity confirmation: LC-MS molecular weight matching the theoretical value

doi: 10.1038/s41419-021-04307-1 107 LiYCaoYXiaoJShangJTanQPingFet al

doi: 10.7554/eLife.15092 110 KrivosovaMGondasEMurinRDohalMOndrejkaITonhajzerovaIet al