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The purpose of this study was to validate hydrodynamic models in the Gulf of Mexico (GOM) by comparing bottom temperature and salinity data with outputs from the MOM6 NWA12 model, in support of CEFI initiatives

Among the most potent of these lipid species are lysophosphatidic acids (LPAs) consisting of a phosphate head group attached to a glycerol backbone and a single FA tail

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The cells in a 96-well plate were incubated in HBSS containing 10 mM FAO blue for 2 h, washed twice with HBSS, and measured using a Spectramax M2 plate reader (excitation/emission = 405 nm/465 nm)

U-2 OS FSP1 GFP-P2A-BFP /RFK KO cells stably expressing Cas9 were transduced with the packaged library as described above

Katsi V et al